In the efficient operation of modern laboratories, the efficiency of liquid transfer and sample handling is crucial. Deep well microplates, as an efficient laboratory tool, offer significant advantages, particularly in terms of sample storage and handling. This article will detail the characteristics, application areas, and precautions when using deep well microplates to help laboratory personnel better utilize this tool to enhance experimental efficiency.
Deep well microplates are designed based on standard microplates (such as 96-well plates, 384-well plates) by increasing the depth of the wells to enhance the volume of each well. They maintain the length and width standards compliant withSBSinternational specifications while improving the depth of the wells. This design makes deep well microplates an ideal choice for sample storage and handling, boosting the efficiency of laboratory operations.
Deep well microplates serve two primary purposes. Firstly, they can be used for sample storage, replacing traditional1.5mlcentrifuge tubes, thus called reservoirs. Secondly, deep well microplates perform excellently in sample handling, especially when used with multichannel pipettes, automated high-throughput liquid handling instruments, and software, facilitating high-throughput operations on biological samples, such as plasmid extraction, protein precipitation, and liquid-liquid extraction, significantly enhancing sample processing efficiency.
Size Compliant with International Standards
Deep well microplates are designed to comply withANSIstandards and internationalSBSstandards, ensuring they fit multichannel pipettes and automated liquid handling systems. This characteristic allows deep well microplates to seamlessly integrate with various automated equipment in high-throughput operations, enhancing operational flexibility and efficiency.
High-Quality Material
Deep well microplates are made fromUSP-Class VIstandard polypropylene (PP) and produced in aGMP10,000cleanroom. The material's resistance to high-temperature autoclaving (121℃,20minutes) and ability to be frozen to-80℃ for storage guarantee stability and reliability during sample processing. PPmateriel also offers excellent chemical resistance, ensuring stability under various experimental conditions.
Precise Design
Deep well microplates feature a mirror-polished interior well design that effectively reduces sample residue. Excellent flatness design ensures the sealing of thermal adhesive films, while the plates can be stacked and feature numeric and alphabetic markings for easy identification and reading. Additionally, deep well microplates can be sealed using adhesive, silicone, or heat sealing to prevent leakage, thus improving operational safety.
Sample Storage
Deep well microplates can replace traditional1.5mlcentrifuge tubes for sample storage. Their large capacity design allows them to store more samples and withstand freezer conditions as low as-80℃. Compared to traditional storage methods, deep well microplates offer a tidy and space-saving storage solution.
Sample Handling
In sample handling, deep well microplates paired with high-throughput automated liquid handling instruments and software allow for efficient high-throughput biological sample processing, such as protein precipitation and liquid-liquid extraction, greatly improving experimental efficiency and reducing complex steps.
When conducting experiments with deep well microplates, consider the following precautions:
Ensure that the irradiated sterilization packaging of deep well microplates is intact to ensure the accuracy of experimental results.
Use specialized multi-channel pipettes and tips to avoid cross-contamination of samples.
Strictly control the volume and concentration of samples to ensure the accuracy of experimental results.
In conclusion, deep well microplates, with their efficient design and diverse application scenarios, have become indispensable assistants in liquid transfer and sample handling in laboratories. Whether for sample storage or high-throughput operations, deep well microplates can significantly improve experimental efficiency and provide robust support for laboratory research.
