Polymerase Chain Reaction (PCR) is a sensitive and efficient method used to amplify a single copy of a target DNA sequence into millions of copies within a short time. Therefore, the plastic consumables used in PCR reactions must be free from contaminants and inhibitors while ensuring high quality that guarantees optimal PCR performance. As a user, it is crucial to understand the characteristics and terminology related to PCR/qPCR consumables so that you can select the most suitable consumables for obtaining accurate and reliable experimental data. Today, let's delve into the key points of PCR consumables.
PCR/qPCR consumables are typically made from polypropylene because it is inert and can withstand the rapid temperature changes during thermal cycling. The inert nature of polypropylene minimizes absorption of reactive substances, ensuring the best possible PCR results. To further ensure batch-to-batch consistency in purity and biocompatibility, virgin, medical-grade, or molecular biology-grade high-quality polypropylene should be used during production. Some PCR/qPCR plate frames are made from stronger polycarbonate to be compatible with high-throughput robotic applications.
PCR tubes and deep well PCR plates are often available in various colors to facilitate visual distinction and identification of samples, especially in high-throughput experiments. Although the color of the plastic does not affect DNA amplification, when setting up real-time PCR or qPCR reactions, we recommend using white or frosted consumables over transparent ones to achieve sensitive and accurate fluorescence detection. White consumables improve the sensitivity and consistency of qPCR data by preventing fluorescence from refracting out of the tubes. Additionally, white tube walls prevent the fluorescent signal from being absorbed or inconsistently reflected by the PCR instrument module, minimizing variations in repeated experiments.
To avoid contamination that could interfere with DNA amplification, PCR plastic consumables should be free from nucleases and DNA contamination. The production of these plastic consumables, from molding to final packaging, must take place in a facility with controlled particle counts to prevent dust and biological contamination.
Manufacturers can provide test reports for PCR/qPCR plastic consumables that have undergone rigorous inspection, ensuring that users obtain accurate, reliable, and reproducible PCR experimental data.
As an environmentally conscious company, MDHC is committed to reducing environmental impact during production and operations. Our PCR consumables are made from biodegradable materials, reducing environmental pollution. We also advocate for green operations in scientific laboratories, promoting sustainable development within the industry. Choosing MDHC's PCR consumables means choosing professional, reliable, and convenient scientific research support. We are dedicated to providing you with the highest quality products and services, helping you achieve more success in your research endeavors.
